The animal studies were approved by the Medical Ethics Committee of Hunan Cancer Hospital (KYJJ-2020-049). Four-week-old BALB/C nude mice purchased from Hunan SJA Laboratory Animal Co., Ltd., were used to establish a xenograft model. SMMC-7721 cells (3 × 106 cells/100 μL per mouse) transfected with miR-10b-5p mimics or NC mimics (GenePharma, China) were subcutaneously injected into the axillae of the anterior left limb of the mice.36 (link) The tumor volumes were measured twice per week. Thirty-five days later, the mice were sacrificed, and tumors were harvested for weighing, imaging, and further detection.
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Xenograft Model of Liver Cancer
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Other organizations : Hunan Cancer Hospital, Central South University
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Variable analysis
independent variables
- MiR-10b-5p mimics
- NC mimics
- Tumor volume
- Tumor weight
- BALB/C nude mice
- SMMC-7721 cells (3 × 10^6 cells/100 μL per mouse)
- Subcutaneous injection into the axillae of the anterior left limb of the mice
- Negative control: NC mimics
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