The C. rodentium strain DBS100 (ATCC 51459; American Type Culture Collection) was used as described69 (link) with some amendments. Briefly, C. rodentium cultures were grown in Luria-Bertani (LB) medium to an OD600 of 1–1.5. Mice were orally inoculated with LB broth or 2 × 109 CFU C. rodentium, and their weight was monitored daily. Fecal samples were collected on indicated days post-infection, serially diluted in PBS and plated on Brilliance™ E. coli/coliform Agar (Thermo Fisher). C. rodentium colonies, from at least three dilutions per sample, were enumerated and normalized to the weight of feces.
For histopathology, 0.5 cm of the distal colon was immediately placed in 10% formalin and processed by the DTU Histology Core for hematoxylin and eosin (H&E) staining. Images were acquired on a Pannoramic MIDI II scanner (3DHISTECH) and visualized with CaseViewer software 2.4 (3DHISTECH). The severity of the infection was assessed in a blinded manner.
For histopathology, 0.5 cm of the distal colon was immediately placed in 10% formalin and processed by the DTU Histology Core for hematoxylin and eosin (H&E) staining. Images were acquired on a Pannoramic MIDI II scanner (3DHISTECH) and visualized with CaseViewer software 2.4 (3DHISTECH). The severity of the infection was assessed in a blinded manner.
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