CHO (Chinese hamster ovary) cells were maintained in Alpha-MEM medium
(Sigma-Aldrich) supplemented with 5% fetal bovine serum (FBS, Gibco) and 2 mM
L-glutamine (Sigma-Aldrich). HEK293 (human embryonic kidney, ATCC) cells were
grown in DMEM (Dulbecco’s modified Eagle’s medium, Sigma-Aldrich)
supplemented with 10% FBS and 2 mM L-glutamine. MDA-MB-231 (triple-negative
human breast adenocarcinoma, ATCC) cells were maintained in DMEM supplemented
with 10% FBS, 2 mM L-glutamine, and 1% non-essential amino acids
(Sigma-Aldrich). Immortalized mouse embryonic fibroblasts (MEFs) isolated from
wild-type and SHARPIN-null (cpdm) mice have been described
previously 7 (link). MEFs were cultured in DMEM
supplemented with 10% FBS, 2 mM L-glutamate, 1 mM sodium pyruvate
(Sigma-Aldrich), 1% non-essential amino acids and 1:100000
β-mercaptoethanol (Sigma-Aldrich). SK-N-BE-2 neuroblastoma cells (ATCC)
were maintained in DMEM:HAM’s F-12 medium supplemented with 10% FBS, 2 mM
glutamine and 1% non-essential amino acids. All cells were routinely tested for
mycoplasma contamination. No cell lines used in this study were found in the
database of commonly misindentified cell lines that is maintained by ICLAC and
NCBI Biosample. The cell lines were not authenticated.