HSP90 inhibitor
compounds, including AT13387 (S1163), AUY922 (S1069), BIIB021 (S1175),
SNX5422 (S2656), and STA9090 (S1159), were purchased from Selleckchem
(Houston, TX) and stored at a stock concentration of 1 mM in DMSO.
Primary Schwann cells were treated with HSP90 inhibitors at the indicated
concentrations in complete media (see above), 24 h after seeding.
DMSO served as the vehicle control while geldanamycin (GA) was used
as a positive control for heat shock pathway activation. The DRG explant
cultures were maintained for 7 days in ascorbate-containing media
prior to treatment with either DMSO, AUY922 (100 nM), or BIIB021 (100
nM), every third day (72 h apart). Cultures were procured, 24 h after
the third treatment, for either biochemical or immunochemical analyses.15 (link)
compounds, including AT13387 (S1163), AUY922 (S1069), BIIB021 (S1175),
SNX5422 (S2656), and STA9090 (S1159), were purchased from Selleckchem
(Houston, TX) and stored at a stock concentration of 1 mM in DMSO.
Primary Schwann cells were treated with HSP90 inhibitors at the indicated
concentrations in complete media (see above), 24 h after seeding.
DMSO served as the vehicle control while geldanamycin (GA) was used
as a positive control for heat shock pathway activation. The DRG explant
cultures were maintained for 7 days in ascorbate-containing media
prior to treatment with either DMSO, AUY922 (100 nM), or BIIB021 (100
nM), every third day (72 h apart). Cultures were procured, 24 h after
the third treatment, for either biochemical or immunochemical analyses.15 (link)
