The peptide profile of the WSEs was analyzed by liquid chromatography–tandem mass spectrometry (LC-MS/MS) in a system consisting of an Agilent 1100 chromatograph (Agilent Technologies, Santa Clara, CA, USA) and an LTQ-FT Ultra mass spectrometer (Thermo Scientific, Waltham, MA, USA) as described in Ref. [13 (link)]. The data are available in Supplementary Materials, Table S1 .
The set analysis of peptidomics data was performed using “UpSetR” and “eulerr” R packages. The heat maps of peptidomics data were constructed using Peptigram—a web-based application for peptidomics data visualization [14 (link)]. The search for peptides with previously reported antioxidant and ACE-I activities was performed in the Milk Bioactive Peptide Database [15 (link)] and BIOPEP-UWM Database [16 (link)].
The set analysis of peptidomics data was performed using “UpSetR” and “eulerr” R packages. The heat maps of peptidomics data were constructed using Peptigram—a web-based application for peptidomics data visualization [14 (link)]. The search for peptides with previously reported antioxidant and ACE-I activities was performed in the Milk Bioactive Peptide Database [15 (link)] and BIOPEP-UWM Database [16 (link)].
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