Quantification of CCoV Infection in A72 Cells
Corresponding Organization : University of Naples Federico II
Other organizations : Istituto Zooprofilattico Sperimentale del Mezzogiorno
Protocol cited in 2 other protocols
Variable analysis
- Pretreatment of A72 cells with CH223191
- Infection of A72 cells with CCoV at MOI of 0.05 and 5
- CCoV quantification by real-time PCR
- Viral cytopathic effect (CPE) evaluation by light microscopy
- Incubation temperature of 37 °C
- Incubation time of 24 hours
- Uninfected A72 cells (negative control)
- Infected A72 cells without CH223191 pretreatment (positive control)
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