Monolayers of A72, pretreated or not with CH223191, were infected or not with CCoV, at an MOI of 0.05 and 5, incubated at 37 °C, and processed after 24 h of infection by real-time PCR for CCoV quantification. Furthermore, viral cytopathic effect (CPE) was evaluated, by examining cells under a light microscope at 24 h p.i. [21 (link),27 (link)].
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