The generation of genetically modified mice Atg7floxp/floxp, Atg5floxp/floxp and Vav-iCre have been previously described [30 (link), 36 (link), 37 (link)]. Vav-iCre mice were purchased from the Jackson laboratory. Breeding and genotyping of mice were also previously described [14 (link)]. Atg7floxp/floxp or Atg5floxp/floxp serves as the control mouse Atg7+/+or Atg5+/+ in this study. All experimental procedures with animals were approved by Soochow University Institutional Animal Care and Use Committee.
Experimental animals Institutional animal care and use committee Mouse
Corresponding Organization :
Other organizations :
First Affiliated Hospital of Soochow University, Soochow University, Tongji Hospital, Tongji University, Second Affiliated Hospital of Soochow University
Generation of genetically modified mice Atg7^floxp/floxp, Atg5^floxp/floxp and Vav-iCre
dependent variables
Not explicitly mentioned
control variables
Atg7^+/+ or Atg5^+/+ mice serve as control mice
controls
Positive control: Atg7^floxp/floxp or Atg5^floxp/floxp mice
Negative control: Atg7^+/+ or Atg5^+/+ mice
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
