Quantifying Cell Invasion Potential

The invasive potential was determined using a 48-well micro chemotaxis Boyden chamber (Neuro Probe, Cabin John, MD, USA). HepG2 and HCCLM3 cells were propagated in a Boyden chamber containing polycarbonate membrane (8-mm pore size) which has been coated with matrigel. Thereafter, the Boyden chamber assay was carried out as reported in our prior reports [59 (link),60 (link)]. The invading cells on the membranes were observed by a Nikon ECLIPSE Ts2 (Nikon corporation, Tokyo, Japan).

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Yang M.H., Mohan C.D., Deivasigamani A., Chinnathambi A., Alharbi S.A., Rangappa K.S., Jung S.H., Ko H., Hui K.M., Sethi G, & Ahn K.S. (2022). Procaine Abrogates the Epithelial-Mesenchymal Transition Process through Modulating c-Met Phosphorylation in Hepatocellular Carcinoma. Cancers, 14(20), 4978.

Publication 2022

Boyden chamber Nikon ECLIPSE Ts2

Assay Cells Chemotaxis Matrigel Membrane Polycarbonate

Corresponding Organization : National University of Singapore

Other organizations : Korea Institute of Science and Technology, University of Mysore, King Saud University

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Variable analysis

independent variables

Cell lines used (HepG2 and HCCLM3)

dependent variables

Invasive potential of the cells

control variables

Boyden chamber containing polycarbonate membrane (8-mm pore size)
Matrigel coating on the membrane
Boyden chamber assay protocol

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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