Chromatin Immunoprecipitation Sequencing

ChIP-Seq was performed as previously described [26 (link)], using anti-H3K27ac (C15410196, Diagenode) or anti-MITF (Sigma, HPA003259). Details of ChIP-seq and data analysis are described in the supplemental information. The human tissue samples used in this study were collected on protocol 2007P000699 “Culture of LAM, AMLs, and other TSC Lesions” approved by the Partners Human Research Committee and informed consent was obtained from all subjects.
Additional methodologic details for metabolomic profiling, whole transcriptome RNA sequencing and immunohistochemistry are provided in supplemental information.

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Zarei M., Giannikou K., Du H., Liu H.J., Duarte M., Johnson S., Nassar A.H., Widlund H.R., Henske E.P., Long H.W, & Kwiatkowski D.J. (2020). MITF is a driver oncogene and potential therapeutic target in kidney angiomyolipoma tumors through transcriptional regulation of CYR61. Oncogene, 40(1), 112-126.

Publication 2020

anti-H3K27ac C15410196 HPA003259

Amls Chip seq Human Immunohistochemistry Mitf Tissue

Corresponding Organization :

Other organizations : Cancer Genetics (United States), Brigham and Women's Hospital, Harvard University, Dana-Farber Cancer Institute, Texas A&M University

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Variable analysis

independent variables

Anti-H3K27ac antibody (C15410196, Diagenode)
Anti-MITF antibody (Sigma, HPA003259)

dependent variables

Genomic regions enriched for H3K27ac and MITF binding (ChIP-Seq data)
Metabolomic profiles
Whole transcriptome gene expression (RNA sequencing)

control variables

Human tissue samples collected under approved protocol 2007P000699

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