S. flexneri 6 OAg was purified and characterized as previously described [17 (link)]. Different lots at average molecular weight of 22 kDa (as estimated by HPLC-SEC analysis) were used, identity was confirmed by 1H NMR and the purity in terms of residual proteins and DNA resulted similar for all lots. S. flexneri 6 OAg glycoconjugate was produced with OAg of same molecular weight by random chemistry as reported in [17 (link)].
Cysteine, Glucose, Galactose, Mannose, Fucose, Galacturonic acid, Rhamnose, Galactosamine, N-acetylglucosamine, KDO, BSA, sodium azide, trifluoroacetic acid, hydrochloric acid were purchased from Sigma-Aldrich (Burlington, MA, USA). Tyvelose was purchased from Toronto Research Chemicals (Toronto, ON, Canada). Sulfuric acid 95–98% was purchased from Merck (Whitehouse Station, NJ, USA). Sodium Hydroxyde 50% was burchased from JT Baker (Radnor, PA, USA).
Cysteine, Glucose, Galactose, Mannose, Fucose, Galacturonic acid, Rhamnose, Galactosamine, N-acetylglucosamine, KDO, BSA, sodium azide, trifluoroacetic acid, hydrochloric acid were purchased from Sigma-Aldrich (Burlington, MA, USA). Tyvelose was purchased from Toronto Research Chemicals (Toronto, ON, Canada). Sulfuric acid 95–98% was purchased from Merck (Whitehouse Station, NJ, USA). Sodium Hydroxyde 50% was burchased from JT Baker (Radnor, PA, USA).
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