Colonic Immunohistochemistry and Immunofluorescence

Immunohistochemistry of colonic sections was performed as described previously [23 (link)]. After microwave antigen retrieval, the colonic slides were incubated overnight with primary antibodies of Ki-67 (Abcam, ab21700, Cambridge, UK) and proliferating cell nuclear antigen (PCNA) (Abcam, ab29, Cambridge, UK) at 4 °C. For immunofluorescence staining, colonic slides were incubated overnight with primary antibodies of β-catenin (Abcam, ab32572, Cambridge, UK) and Muc-2 (Abcam, ab272692, Cambridge, UK) at 4 °C.

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Li D., Feng Y., Tian M., Hu X., Zheng R, & Chen F. (2021). Dietary Barley Leaf Mitigates Tumorigenesis in Experimental Colitis-Associated Colorectal Cancer. Nutrients, 13(10), 3487.

Publication 2021

ab21700 ab32572 ab272692

Antibodies Antigen Colonic Immunofluorescence Immunohistochemistry Microwave Muc 2 Proliferating cell nuclear antigen β catenin

Corresponding Organization : Peking University

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Variable analysis

independent variables

Primary antibodies of Ki-67 (Abcam, ab21700, Cambridge, UK) and proliferating cell nuclear antigen (PCNA) (Abcam, ab29, Cambridge, UK)
Primary antibodies of β-catenin (Abcam, ab32572, Cambridge, UK) and Muc-2 (Abcam, ab272692, Cambridge, UK)

dependent variables

Immunohistochemistry and immunofluorescence staining of colonic sections

control variables

Microwave antigen retrieval
Incubation of colonic slides with primary antibodies at 4 °C overnight

controls

Positive control: Not specified
Negative control: Not specified

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