Influenza Vaccination and Pneumococcal Colonization

Healthy adult volunteers were 1:1 randomized to receive either intranasally LAIV (2015/2016 Fluenz Tetra or FluMist Tetra, AstraZeneca, UK) or intramuscular Quadrivalent Inactivated Influenza Vaccination (Fluarix Tetra, GlaxoSmithKline, UK) as described previously 14 (link). The control group also received a nasal saline spray, while the LAIV group also received a intramuscular saline injection. Three days post vaccination all subjects were inoculated with 80,000 CFU per nostril of 6B type Spn as described 6 (link),51 (link). Nasal microbiopsies (ASL Rhino-Pro^©, Arlington Scientific) and nasal lining fluid (Nasosorption™, Hunt Developments) samples were collected and stored at -80C as previously described 52 (link).

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Jochems S.P., Marcon F., Carniel B.F., Holloway M., Mitsi E., Smith E., Gritzfeld J.F., Solórzano C., Reiné J., Pojar S., Nikolaou E., German E.L., Hyder-Wright A., Hill H., Hales C., de Steenhuijsen Piters W.A., Bogaert D., Adler H., Zaidi S., Connor V., Gordon S.B., Rylance J., Nakaya H.I, & Ferreira D.M. (2018). Inflammation induced by influenza virus impairs human innate immune control of pneumococcus. Nature Immunology, 19(12), 1299-1308.

Publication 2018

Fluenz Tetra FluMist Tetra Fluarix Tetra

Adult Fluarix Flumist Healthy volunteers Influenza Intramuscular injection Nasal Nasal spray Saline Tetra Vaccination

Corresponding Organization : Liverpool School of Tropical Medicine

Other organizations : Universidade de São Paulo, Royal Liverpool and Broadgreen University Hospital NHS Trust, Centre for Inflammation Research, University of Edinburgh

Top 5 similar protocols

Variable analysis

independent variables

Intranasally LAIV (2015/2016 Fluenz Tetra or FluMist Tetra, AstraZeneca, UK)
Intramuscular Quadrivalent Inactivated Influenza Vaccination (Fluarix Tetra, GlaxoSmithKline, UK)

dependent variables

Nasal microbiopsies (ASL Rhino-Pro©, Arlington Scientific)
Nasal lining fluid (Nasosorption™, Hunt Developments)

control variables

Nasal saline spray
Intramuscular saline injection

positive controls

None specified

negative controls

Nasal saline spray

Annotations

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