Neutrophil and Reticulocyte Isolation
Corresponding Organization :
Other organizations : Université Paris Cité, Inserm, Assistance Publique – Hôpitaux de Paris, University of Michigan–Ann Arbor, Centre Hospitalier Universitaire de Grenoble, Université Grenoble Alpes, Établissement Français du Sang, Queen Fabiola Children's University Hospital, Centre Hospitalier Universitaire Brugmann, Université Libre de Bruxelles, Institut des Maladies Génétiques Imagine, New York Blood Center, Grenoble Institute of Neurosciences, Centre National de la Recherche Scientifique
Variable analysis
- Neutrophil isolation using MACSxpress Neutrophil Isolation Kit
- Reticulocyte isolation using CD71 MicroBeads Kit
- Purity of isolated neutrophils and reticulocytes, determined by flow cytometry using anti-CD16 and anti-CD71 antibodies
- Time between blood sampling and neutrophil isolation (< 4 h)
- Whole blood as starting material for both neutrophil and reticulocyte isolation
- No positive or negative controls specified
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