Human neutrophils were isolated from fresh whole blood (< 4 h after blood sampling) using the MACSxpress Neutrophil Isolation Kit followed by a MACSxpress Erythrocyte Depletion Kit (Miltenyi Biotec). Reticulocytes were isolated from whole blood using the CD71 MicroBeads Kit (Miltenyi Biotec). The purity of the cells, determined by flow cytometry using anti‐CD16 and anti‐CD71 antibodies, was approximately 98%.
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