Western Blotting for Cellular Signaling

Western blotting was performed as previously described (10 (link)). We used the following primary antibodies: rabbit polyclonal antibody specific for PARP, phospho-TBK1/NAK (Ser¹⁷²), TBK1, phospho-polo-like kinase-1 (PLK1) (Thr²¹⁰), and PLK1from Cell Signaling (Beverly, MA), and β-actin antibody from Sigma (St. Louis, MO).

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Li J., Fang B., Kinose F., Bai Y., Kim J.Y., Chen A., Rix U., Koomen J.M, & Haura E.B. (2016). Target identification in small cell lung cancer via integrated phenotypic screening and activity-based protein profiling. Molecular cancer therapeutics, 15(2), 334-342.

Publication 2015

PLK1) (Thr210), β-actin antibody

Actin Antibodies Antibody Polo like kinase 1 Rabbit Tbk1

Corresponding Organization : Moffitt Cancer Center

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Variable analysis

independent variables

Primary antibodies used: rabbit polyclonal antibody specific for PARP, phospho-TBK1/NAK (Ser172), TBK1, phospho-polo-like kinase-1 (PLK1) (Thr210), and PLK1 from Cell Signaling (Beverly, MA), and β-actin antibody from Sigma (St. Louis, MO)

dependent variables

Not explicitly mentioned

control variables

Western blotting was performed as previously described (10 (link))

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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