Quantifying Intestinal Autofluorescence in Nematodes

Intestinal autofluorescence caused by lysosomal deposits of lipofuscin can accumulate over time in aging nematodes or nematodes exposed to specific toxicants, and the analytical method was performed as described previously [37 (link),38 (link)]. The exposed nematodes were anaesthetised with levamisole solution (60 µM), and then pipetted onto a slide containing a 2% agarose pad. Fluorescence intensity images of each group of nematodes intestinal autofluorescence were taken under a fluorescence microscope (UV-2A filter). The images were analysed by ImageJ V1.8.0 software and the results were expressed as mean fluorescence intensity values. A minimum of 30 nematodes were detected in each group, and the process was repeated three times.

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Yang R., Ge P., Liu X., Chen W., Yan Z, & Chen M. (2023). Chemical Composition and Transgenerational Effects on Caenorhabditis elegans of Seasonal Fine Particulate Matter. Toxics, 11(2), 116.

Publication 2023

Agarose Fluorescence Fluorescence microscope Intestinal Levamisole Lipofuscin Lysosomal Nematodes

Corresponding Organization : Nanjing University of Information Science and Technology

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Variable analysis

independent variables

Exposure to specific toxicants

dependent variables

Intestinal autofluorescence intensity

control variables

Anaesthesia with levamisole solution (60 µM)
Nematodes placed on 2% agarose pad

controls

Positive control: Unexposed nematodes (aging nematodes)
Negative control: Not mentioned

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