Cytokine and Enzymatic Profiling

Levels of the serum cytokines TNF-α, IL-1β, and IL-6 were measured by the Cytokine Reference Lab at the University of Minnesota (MAGPIX, Luminex, Austin, TX, USA). Amylase (Abcam, ab102523), lipase (Sigma-Aldrich, MAK046), and LDH (Sigma-Aldrich, MAK066) activity assays were performed according to kit instructions. Western blotting was performed in pancreatic lysates as previously described [28 (link)]. Antibodies against OGT and Vinculin were purchased from Cell Signaling Technology.

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Moore M., Avula N., Wong A., Beetch M., Jo S, & Alejandro E.U. (2022). Reduction in O-GlcNAcylation Mitigates the Severity of Inflammatory Response in Cerulein-Induced Acute Pancreatitis in a Mouse Model. Biology, 11(3), 347.

Publication 2022

MAK046

Amylase Antibodies Assays Austin Cytokine Il 1β Lipase Pancreatic Serum Tnf α Vinculin

Corresponding Organization : University of Minnesota Medical Center

Other organizations : University of Minnesota

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Variable analysis

independent variables

Levels of the serum cytokines TNF-α, IL-1β, and IL-6

dependent variables

Amylase activity
Lipase activity
LDH activity
Protein expression of OGT and Vinculin in pancreatic lysates

control variables

Not explicitly mentioned

controls

Positive and negative controls not explicitly mentioned

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