Western blot analysis was performed as previously described (17 (link)). Briefly, total protein was isolated and quantitated using BCA assay at 48 h post-transfection. The protein lysates (40 µg) were electrophoretically transferred onto PVDF membranes (EMD Millipore), followed blocking with 5% skim milk at 4°C overnight. The membranes were probed with primary antibodies against EZH2 (1:1,000; cat. no. 5246), E-cadherin (1:1,000; cat. no. 3195), N-cadherin (1:1,000; cat. no. 13116) and β-actin (1:1,000; cat. no. 4970), followed by incubation with secondary antibody (anti-rabbit IgG, 1:10,000; cat. no. 7074) for 1 h at room temperature. All antibodies were obtained from Cell Signaling Technology, Inc. Proteins bands were detected using an ECL detection system (GE Healthcare Life Sciences) and blot bands were quantified using ImageJ software (version 1.46; Rawak Software, Inc.).