Dissociated primary cortical cultured neurons were prepared from P0 C57BL/6J (The Jackson Laboratory). Brains were dissected in ice-cold Leibowitz’s L-15 media with penicillin/streptomycin, and cortical tissue isolated, digested with 0.25% trypsin-EDTA solution at 37°C for 10 min, and mechanically dissociated in high-glucose Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% FBS, 1.4 mM L-glutamine, and 6.0 g/L glucose. The detailed information was described previously26 (link), 27 (link). All procedures were in compliance with the National Institutes of Health standards and were approved by Northwestern University’s Animal Care and Use Committee.