Isolation and Characterization of Primary Human Trabecular Meshwork Cells

Primary HTM cells were obtained from ScienCell Research Laboratories (Carlsbad, CA, USA). HTM cells were isolated from a single donor. To characterize these HTM cells, we confirmed myocilin (MYOC) upregulation induced by dexamethasone (DEX) treatment according to the procedure described in previous reports [31 (link)–34 (link)] (S1 Fig). HTM cells were maintained in the supplemented manufacturer-specified medium, namely, trabecular meshwork cell medium (TMCM) containing 2% fetal bovine serum, the manufacturer-specified supplement (i.e., undisclosed growth factors, hormones, and proteins), 100 units/mL of penicillin, and 100 μg/mL of streptomycin (ScienCell Research Laboratories). HTM cells were cultured in a poly-L-lysine-coated flask, and the supplemented TMCM was exchanged every 3–4 d. HTM cells were used between passages 3 and 5 in the present study.