Hypoxic Exposure and HIF-1α Detection

Hypoxic exposure of PASMC, protein extraction, and Western blot analysis were performed as described (5 (link)). Primary antibodies used were anti–HIF-1α (1:1000 dilution; Cayman Chemical) and anti–β-actin (1:50,000 dilution; Abcam). Specific immune-reactive signals were detected using enhanced chemiluminescence ECL Prime Western Blotting System (GE Healthcare) using a proprietary secondary antibody coupled to horseradish peroxidase (1:5000 dilution; Promega).

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Sommer N., Alebrahimdehkordi N., Pak O., Knoepp F., Strielkov I., Scheibe S., Dufour E., Andjelković A., Sydykov A., Saraji A., Petrovic A., Quanz K., Hecker M., Kumar M., Wahl J., Kraut S., Seeger W., Schermuly R.T., Ghofrani H.A., Ramser K., Braun T., Jacobs H.T., Weissmann N, & Szibor M. (2020). Bypassing mitochondrial complex III using alternative oxidase inhibits acute pulmonary oxygen sensing. Science Advances, 6(16), eaba0694.

Publication 2020

anti–HIF-1α anti–β-actin ECL Prime Western Blotting System horseradish peroxidase

Actin Antibodies Antibody Cayman Chemiluminescence Dilution Horseradish peroxidase Hypoxic Promega Protein Western blot analysis

Corresponding Organization :

Other organizations : Cardio-Pulmonary Institute, University of Giessen, Universities of Giessen and Marburg Lung Center, German Center for Lung Research, Tampere University, Luleå University of Technology, Hammersmith Hospital, Imperial College London, Max Planck Institute for Heart and Lung Research

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Variable analysis

independent variables

Hypoxic exposure of PASMC

dependent variables

Protein expression levels of HIF-1α

control variables

Protein expression levels of β-actin

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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