Seventy-two hours after irradiation, cells and media were collected, centrifuged, and resuspended in Annexin binding buffer with cell density adjusted to ~106/ml. Cells were stained with propidium iodide (Sigma-Aldrich) and Annexin V-Cy5 following the manufacturer’s protocol (BioVision, Milpitas, CA), and then analyzed by a LSRII flow cytometer (BD Biosciences, San Jose, CA). Senescence-associated β-galactosidase staining was performed using a commercial kit (Cell Signaling, #9860) as described (23 (link)).
Protocol detail
Quantifying Irradiation-Induced Apoptosis
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Harvard University
Other organizations : Shandong Center for Disease Control and Prevention, Center for Cancer Research
Top 5 similar protocols
Variable analysis
independent variables
- Irradiation
- Annexin V-Cy5 staining
- Propidium iodide staining
- Senescence-associated β-galactosidase staining
- Cell density adjusted to ~10^6/ml
- Annexin binding buffer
- Propidium iodide (Sigma-Aldrich)
- Annexin V-Cy5 (BioVision, Milpitas, CA)
- LSRII flow cytometer (BD Biosciences, San Jose, CA)
- Senescence-associated β-galactosidase staining kit (Cell Signaling, #9860)
- Not explicitly mentioned
- Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!