Peptide Screening and Characterization for PEDV

The peptides were screened by molecular docking in our laboratory by SYBYL-X 2.0 software, synthesized, and purified to at least 90% by GL Biochem (Shanghai, China). The porcine epidemic diarrhea virus S protein (PEDV-S1), PEDV S1 protein monoclonal antibody, anti-his tag mouse monoclonal antibody were made and PEDV N protein monoclonal was conserve in our laboratory, porcine circovirus 2 Cap protein (PCV2-Cap), porcine reproductive and respiratory syndrome virus GP5 protein (PRRSV-GP5) were generated in our laboratory. TransZol Up and PerfectStartIIProbe qPCR SuperMix UDG were purchased from TransGen Biotech (Beijing China). The goat anti-mouse IgG conjugated to horseradish peroxidase (IgG-HRP) was purchased from Abbkine (Wuhan, China) antibody goat anti-mouse IgG(H+L) highly cross-adsorbed secondary antibody, Alexa Fluor Plus 488, was purchased by Thermo Fisher Scientific (Invitrogen, Waltham, MA, USA). The cell-counting kit-8 (CCK-8), 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI) and RIPA lysis buffer were purchased from Beyotime (Shanghai, China).