Western Blot Analysis of Nuclear Proteins

For Western analysis, immunoprecipitated nuclear extracts were separated by 10% sodium dodecyl sulfate (SDS)-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Immobilon FL, Millipore). The following primary antibodies were used for immunoblotting: anti-REST antibody (Millipore, 17–641), anti-HDAC1 (Abcam, ab7028), anti-HDAC2 (Abcam, ab51832), anti-LSD1 (Abcam, ab17721), anti-NME2 (Abcam, ab60602) (23 (link), 24 (link), 40 (link)), and anti-hTERT (Abcam, ab32020) (49 (link), 50 ). The secondary antibodies used were anti-mouse and anti-rabbit alkaline phosphatase conjugates from Sigma. Western blots with antibodies against NME2, telomerase, REST, and LSD1 along with relevant molecular weight markers are shown in supplemental Fig. S5.