Cells were lysed in RIPA buffer supplemented with Complete Protease Inhibitor Cocktail and PhosphoStop (both from Roche, Mannheim, Germany) and Western blot analyses were performed as described previously [22 (link)]. Primary antibodies were purchased from: Cell Signaling, Frankfurt, Germany (anti-ERK1/2 (9102), anti-phospho-ERK1/2 (9106), anti-JNK (9252), anti-phospho-JNK (9251), anti-p38 (9217), anti-phospho-p38 (9211), anti-caspase-8 (9746S), anti-caspase-3 (9668), anti-caspase-9 (9502), anti-decoy receptor 2 (8049) and anti-PARP-1 (9542)); Santa Cruz Biotechnology, Heidelberg, Germany (anti-p53 DO-1 (sc-126)); BD Transduction Laboratories, Heidelberg, Germany (anti-Bax (610982) and anti-APAF1 (A92820)); Merck Millipore, Darmstadt, Germany (anti-DR4 (AB16955)); ProScience Incorporated, USA (anti-TRAIL-R2 (2019)); R&D Systems, Wiesbaden, Germany (anti-BID (AF846)); Abcam, Cambridge, UK (anti-TRAIL-3 (AB133658)) and from Sigma-Aldrich (anti-β-actin (A5441)).
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