Histological Assessment of Cardiac Fibrosis and Hypertrophy

After euthanizing the mice, their hearts were extracted and subjected to a semi-quantitative assessment of fibrosis and cellular hypertrophy, following [12 (link)]. The samples were stained with Masson’s Trichrome or hematoxylin and eosin, and microphotographs were captured using an Imager Z.1 Zeiss microscope equipped with an AxioCam HRm camera. The microphotographs were processed using the AxioVision software. The PV records of mice with hearts that showed no signs of fibrosis and cellular hypertrophy indicative of heart failure were excluded from the study.

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Fernández-Mendoza G., Méndez-Fernández A., Alves-Figueiredo H.J., García-Rivas G, & Santillán M. (2023). Exploring the mechanisms underlying stroke volume variability reduction in a murine model of heart failure with reduced ejection fraction. PLOS ONE, 18(10), e0292687.

Publication 2023

Imager Z.1 Zeiss microscope AxioCam HRm camera

Cellular hypertrophy Eosin Fibrosis Heart failure Hearts Hematoxylin Mice Microscope

Corresponding Organization : Centro de Investigación en Materiales Avanzados

Other organizations : Instituto Politécnico Nacional, Tecnológico de Monterrey

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Variable analysis

independent variables

Euthanizing the mice

dependent variables

Fibrosis assessment
Cellular hypertrophy assessment

control variables

Staining method (Masson's Trichrome or hematoxylin and eosin)
Microscope used (Imager Z.1 Zeiss microscope equipped with an AxioCam HRm camera)
Image processing software (AxioVision)

controls

Positive control: Hearts that showed signs of fibrosis and cellular hypertrophy indicative of heart failure
Negative control: Hearts that showed no signs of fibrosis and cellular hypertrophy indicative of heart failure (excluded from the study)

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