Microscopic Identification of Algae Genera and Species

Microscopic morphologic identification at the genus level was performed according to Bellinger & Sigee, 2015 [56 ]. Further identification to species levels was accomplished by comparison with the species original descriptions that are available at the AlgaeBase [57 ]. In the case of the as of yet undescribed species, the morphological comparisons were made with the closest strains obtained in the molecular identification step: Desmodesmus sp. MAT2008c [58 (link)]; Micractinium sp. CCAP 211/92 [39 (link)]; Desmodesmus sp. GM4a [59 (link)]. A Carl Zeiss Axio Imager A2 microscope (Zeiss.co, Brazil) equipped with Differential Interference Contrast (DIC) was used for morphological analysis.

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Hadi S.I., Santana H., Brunale P.P., Gomes T.G., Oliveira M.D., Matthiensen A., Oliveira M.E., Silva F.C, & Brasil B.S. (2016). DNA Barcoding Green Microalgae Isolated from Neotropical Inland Waters. PLoS ONE, 11(2), e0149284.

Publication 2016

Carl Zeiss Axio Imager A2 microscope

Ccap Microscope Strains

Corresponding Organization : Universidade Federal da Bahia

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Variable analysis

independent variables

Microscopic morphologic identification at the genus level
Further identification to species levels by comparison with the species original descriptions

dependent variables

Morphological comparisons with the closest strains obtained in the molecular identification step

control variables

A Carl Zeiss Axio Imager A2 microscope (Zeiss.co, Brazil) equipped with Differential Interference Contrast (DIC) was used for morphological analysis

controls

No positive or negative controls were explicitly mentioned in the provided information.

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