Inhibiting C-type Lectin Receptors with Mannan

The antibodies used in the experiments were Mouse IgG2a Isotype Control (Invitrogen), MERS Coronavirus Spike Protein neutralizing Antibody MA5-29975 (Invitrogen), Invitrogen CD26 Monoclonal Antibody (BA5b) (Invitrogen), Human DC-SIGN+DC-SIGNR Antibody (R&D Systems), PE anti-human CD209 (DC-SIGN) Antibody (Biolegend), and CD26 Monoclonal Antibody (BA5b) PE (Invitrogen). As a lectin-binding control, mannan from Saccharomyces cerevisiae (Sigma-Aldrich) was used. Mannan is used in experiments to inhibit C-type lectin receptors, such as DC-SIGN due to its ability to block the interaction between these receptors and viral or pathogenic ligands. Being a complex polysaccharide, mannan competes with the binding sites of the receptors, acting as a decoy and preventing the attachment of infectious agents to host cells (Jan et al., 2018 (link)).