Comprehensive Cocoa Metabolite Profiling

A non-targeted screening through headspace/solid-phase microextraction coupled to gas chromatography with time-of-flight mass spectrometry (HS/SPME-GC-TOF-MS) of the ground cocoa pulp, cocoa beans, and chocolate-making samples was conducted, in triplicate, using a Trace 1300 gas chromatograph (Thermo Fisher Scientific) equipped with a Stabilwax-MS column (Restek), as described previously (Díaz-Muñoz et al., 2021 (link)). This metabolite fingerprinting technique served as a selection of VOCs to be quantified.

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Van de Voorde D., Díaz-Muñoz C., Hernandez C.E., Weckx S, & De Vuyst L. (2023). Yeast strains do have an impact on the production of cured cocoa beans, as assessed with Costa Rican Trinitario cocoa fermentation processes and chocolates thereof. Frontiers in Microbiology, 14, 1232323.

Publication 2023

Stabilwax-MS column

Chocolate Cocoa Gas chromatograph Gc ms Mass spectrometry Pulp Spme

Corresponding Organization : Vrije Universiteit Brussel

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Variable analysis

independent variables

Ground cocoa pulp
Cocoa beans
Chocolate-making samples

dependent variables

Volatile organic compounds (VOCs)

control variables

Headspace/solid-phase microextraction coupled to gas chromatography with time-of-flight mass spectrometry (HS/SPME-GC-TOF-MS) analysis
Trace 1300 gas chromatograph (Thermo Fisher Scientific) equipped with a Stabilwax-MS column (Restek)

controls

The study was conducted in triplicate, which serves as a form of technical replication to ensure the validity of the results.

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