For northern blotting, 10–30 μg of RNA was separated by denaturating polyacrylamide gels (7 M urea) and subsequently electro-blotted onto nylon membranes (Amersham Hybond N+, GE Healthcare) as described [13 (link)]. All membranes were hybridized to a 32P-5′-end-labelled DNA probe complementary to the 5ʹ part of tRNAPro AATCATACCCCTAGACCAACGAGCC.
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