rCRD was suspended in phosphate-buffered saline (PBS) (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, and 1.8 mM KH2PO4, pH 7.4), and injected into 6-week-old mice to generate polyclonal antibodies, following the procedure outlined by Pan et al. [13 (link)]. The IgG of the rCRD antibody was purified using protein A agarose (Beyotime, China). The specificity of the antibody was detected by Western blot analysis [14 (link)].
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