Cell viability was assessed using a 3-(4, 5-dimethylthiazol-2-yl)-2,
5-diphenyltetrazo-lium bromide (MTT) assay. Cells (5000/well) in 100 µL complete
media were planted into 96-well plate. The next day cells were exposed to serial
dilutions of GGLE for 48 hours. Then 20 μL of 5 mg/mL MTT were added into each
well, and the plates were incubated in the cell culture incubator for 4 hours.
The colorimetric MTT assay assesses relative proliferation, based on the ability
of living cells to reduce MTT to formazan. The formazan crystal was dissolved in
150 μL/well dimethyl sulfoxide (DMSO) and color was measured at 570 nm using a
microplate reader (BioTek Instruments, Inc.). The 50% inhibitory concentration
(IC50) was defined as the concentration of drug that inhibited
cell growth by 50% relative to the untreated control.
5-diphenyltetrazo-lium bromide (MTT) assay. Cells (5000/well) in 100 µL complete
media were planted into 96-well plate. The next day cells were exposed to serial
dilutions of GGLE for 48 hours. Then 20 μL of 5 mg/mL MTT were added into each
well, and the plates were incubated in the cell culture incubator for 4 hours.
The colorimetric MTT assay assesses relative proliferation, based on the ability
of living cells to reduce MTT to formazan. The formazan crystal was dissolved in
150 μL/well dimethyl sulfoxide (DMSO) and color was measured at 570 nm using a
microplate reader (BioTek Instruments, Inc.). The 50% inhibitory concentration
(IC50) was defined as the concentration of drug that inhibited
cell growth by 50% relative to the untreated control.
