Erythroleukemia Cell Line HEL: Drug Treatment and Proliferation Analysis

The erythroleukemia cell line HEL (human origin) was originally obtained from ATCC (HEL 92.1.7), and maintained mycoplasma free used in all experiments.19 (link),20 (link) The generation of inducible K562-fli1 cells is described elsewhere.20 (link) These cells were maintained in Dulbecco’s Modified Eagle Medium supplemented with HyClone 5% fetal bovine serum (GE Healthcare).
The HEL cells were treated with indicated drugs, used for cell counting/MTT assay (to determine the proliferation index) and for protein/mRNA extraction (for Western blotting and RT-qPCR). Famotidine was purchased from (APExBIO), Tetrandrine from (Solarbio, CN), EGC from (Selleckchem, CN) and Diacerein from (APExBIO).

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Hu J., Gao J., Wang C., Liu W., Hu A., Xiao X., Kuang Y., Yu K., Gajendran B., Zacksenhaus E., Pan W, & Ben-David Y. (2023). FLI1 Regulates Histamine Decarboxylase Expression to Control Inflammation Signaling and Leukemia Progression. Journal of Inflammation Research, 16, 2007-2020.

Publication 2023

HyClone 5% fetal bovine serum Tetrandrine

Assay Cell line Cells Diacerein Drugs Eagle Erythroleukemia Famotidine Fetal bovine serum K562 cells Mrna Mycoplasma Protein Tetrandrine

Corresponding Organization :

Other organizations : Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang Medical University, University Health Network, University of Toronto

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Variable analysis

independent variables

Famotidine
Tetrandrine
Diacerein

dependent variables

Cell proliferation (determined by cell counting/MTT assay)
Protein expression (determined by Western blotting)
MRNA expression (determined by RT-qPCR)

control variables

HEL (human erythroleukemia) cell line
Mycoplasma-free cell culture conditions
Dulbecco's Modified Eagle Medium supplemented with 5% fetal bovine serum

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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