Profiling Differential circRNAs in Mouse Aorta

Total RNA was extracted from mouse aortic tissue using TRIzol LS Reagent (Invitrogen life technologies), RNA concentration and purity were determined by NanoDrop® ND-1000, and the RNase R-treated samples were subjected to labeling reactions and array hybridization [11 (link)]. The data were scanned with an Agilent Scanner G2505 C (Agilent, Santa Clara, CA, USA) using an Arraystar version 2.0 mouse circRNA microarray, the raw data were read by Agilent Feature Extraction software (v11.0.1.1) (Agilent, Santa Clara, CA, USA.), and the R software limma package normalizes the qualified data of quality control and analyzes the standardized gene chip data to obtain differential circRNAs expression profile. CircRNAs were identified to be differentially expressed (fold change >1.5; P < 0.05) [12 (link)].