HBEpCs were grown on chamber slides (Chamber slide, Lab-TekII; Thermo Fisher Scientific, Rochester, NY) and infected with H1N1 or H3N2 for 2–6 h. Confocal staining of the cells was done as described earlier (25 (link)). Cells were treated with rabbit anti-human FADD or DR3 antibody (Cell Signaling) and anti-mouse TRADD (Millipore, Billerica, MA) followed by a secondary Alexa-488 conjugated anti-rabbit antibody and Alexa-546 conjugated anti-mouse antibody (Invitrogen).
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Confocal Staining of Virus-Infected Cells
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Corresponding Organization : Centers for Disease Control and Prevention
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Variable analysis
independent variables
- H1N1 infection
- H3N2 infection
- Localization of FADD
- Localization of DR3
- Localization of TRADD
- HBEpCs (human bronchial epithelial cells) used as the cell line
- Cells grown on chamber slides
- Cells stained using confocal microscopy as described in a previous study (25)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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