Western Blot Analysis of INTS7 and ABCD3

Western blot analysis was performed as previously described with a minor change (Wu et al., 2021 (link)). In short, 48 h after inhibition using Ints7 MO oligos, BM-MSC protein lysates were separated by 10% sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to 0.22-μm polyvinylidene difluoride membranes (Millipore, Billerica, MA, United States) and incubated with specific anti-INTS7 (Proteintech, Chicago, IL, United States) or anti-ABCD3 (Abcam, Cambridge, MA, United States) antibodies. A beta-tubulin antibody (Beyotime, Nantong, China) was used as an internal control. After incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Thermo Scientific, Waltham, MA, United States), signals were detected by enhanced chemiluminescence substrate (Thermo Scientific). The resulting bands were analyzed using Image-Pro Plus Software.