Diabetic Akita Mouse Model Study

Male wild-type C57BL/6J mice (stock no. 000664) and diabetic C57BL/6-Ins2^Akita/J mice (stock no. 003548) of 10–14 weeks old were obtained from the Jackson Laboratory (Bar Harbor, ME). Mice were fed standard mouse chow and water ad libitum, and were maintained under a 12:12 h light–dark cycle. All animal experiments were carried out in accordance with the institutional animal care and use committee-approved protocols (approval no. 20683, dated 2 December 2020) of the University of Louisville School of Medicine and conformed to the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health (NIH Publication, 2011), U.S.A. Wild-type and diabetic Akita mice were treated either with normal saline or with GYY (0.25 mgKg⁻¹ d⁻¹, I.P.) for 8 weeks [13 (link),58 (link),59 (link)]. At the end of the experiment, mice were euthanized by using 2X tribromoethanol (TBE), and both blood and kidney samples were collected.