Characterization of Murine Pancreatic Tumor Clones
The clonal cell lines described (E, H, V, K, M, N and T) were isolated from pancreatic tumors from KPC mice and have been previously described30 . Clonal lines 6419c5, 6694c2, 2838c3 and 6499c4 were derived from KPC mice and have also been previously described24 (link). KPC7940B cells were a gift from G. Beatty (University of Pennsylvania). KPC-MT3 cells were a gift from D. Tuveson (Cold Spring Harbor Laboratory). PATC53 cells were obtained from ATCC. Cells were maintained in high-glucose DMEM (Gibco) supplemented with 10% fetal bovine serum (Corning) and routinely tested for mycoplasma contamination using MycoAlert Plus (Lonza). DMSO, oligomycin, phenformin and aminooxyacetic acid were obtained from Sigma, trans-ISRIB was obtained from Cayman Chemical and GCN2iB was obtained from MedChemExpress. IACS-10759 was obtained from Selleck Chem. KPC clonal lines were genotyped for KrasG12D recombination using primer sets from Jackson Laboratories.
Corresponding Organization : University of California, Irvine
Other organizations :
University of Michigan–Ann Arbor, Cancer Research UK Manchester Institute, University of Manchester, University of California, Los Angeles, Riley Hospital for Children, Indiana University Health, Indiana University – Purdue University Indianapolis, Cancer Research UK Scotland Institute, University of Glasgow, University of Pennsylvania, The University of Texas MD Anderson Cancer Center
Cell lines (E, H, V, K, M, N, T, 6419c5, 6694c2, 2838c3, 6499c4, KPC7940B, KPC-MT3, PATC53)
control variables
High-glucose DMEM (Gibco) supplemented with 10% fetal bovine serum (Corning)
Cells routinely tested for mycoplasma contamination using MycoAlert Plus (Lonza)
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