OXTR Gene Genotyping for Sociality

DNA was collected from peripheral blood using a QIAamp DNA Blood Kit (Qiagen, Tokyo, Japan). To detect the rs53576 of the OXTR gene, TaqMan SNP Genotyping Assay (C_3290335_20; Thermo Fisher Scientific) and TaqPath Master Mix (Thermo Fisher Scientific) were used. For this study, the OXTR genotypes were divided into two groups: A allele carriers (A/A + A/G) and A allele noncarriers (G/G), according to earlier meta‐analyses showing that the A allele is dominantly related to sociality (Li et al., 2015 (link)) and empathy (Gong et al., 2017 (link)).

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Noto K., Suzuki A., Shirata T., Matsumoto Y., Muraosa H., Goto K, & Otani K. (2021). Oxytocin receptor polymorphism influences characterization of harm avoidance by moderating susceptibility to affectionless control parenting. Brain and Behavior, 11(11), e2393.

Publication 2021

QIAamp DNA Blood Kit TaqMan SNP Genotyping Assay TaqPath Master Mix

Allele Assay Blood Gene Genotypes

Corresponding Organization : Yamagata University

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Variable analysis

independent variables

OXTR genotypes (A allele carriers (A/A + A/G) and A allele noncarriers (G/G))

dependent variables

Not explicitly mentioned

control variables

Peripheral blood samples collected using a QIAamp DNA Blood Kit (Qiagen, Tokyo, Japan)
TaqMan SNP Genotyping Assay (C_3290335_20; Thermo Fisher Scientific) used to detect the rs53576 of the OXTR gene
TaqPath Master Mix (Thermo Fisher Scientific) used

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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