Quantitative Lipidomics Analysis Protocol

Methanol, 2-propanol, dichloromethane, water, ammonium-acetate were purchased from VWR International, LLC (Radnor, Radnor, PA, USA). All of them were of HPLC grade. Internal standard (ISTD) kits for quantitative lipidomic analysis of human samples were bought from AB Sciex Germany GmbH (Darmstadt, Germany). The kits contain ISTDs for 13 lipid classes including ceramides (Cers), cholesterolesters (CEs), DGs, acylceradmides (ACer), fatty acids (FAs), hexosylceramides (HexCers), lactosylceramides (LacCers), LPCs, LPEs, PCs, phosphatidylethanolamines (PEs), SMs, and TGs. The composition of ISTD standard mixtures containing isotope-labelled lipid molecules were described in detail previously [18 (link)]. Spike standards with quality control plasma kits, SelexION tuning kits, and system suitability test kits were also obtained from AB Sciex Germany GmbH.

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Pikó P., Pál L., Szűcs S., Kósa Z., Sándor J, & Ádány R. (2021). Obesity-Related Changes in Human Plasma Lipidome Determined by the Lipidyzer Platform. Biomolecules, 11(2), 326.

Publication 2021

Methanol 2-propanol ammonium-acetate SelexION tuning kits system suitability test kits

2 propanol Ammonium acetate Ceramides Dichloromethane Fatty acids Hplc Human Isotope Lactosylceramides Lipid Methanol Phosphatidylethanolamines Plasma

Corresponding Organization : University of Debrecen

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Variable analysis

independent variables

Methanol
2-propanol
Dichloromethane
Water
Ammonium-acetate

dependent variables

Quantitative lipidomic analysis of human samples

control variables

All chemicals were of HPLC grade

controls

Positive control: Internal standard (ISTD) kits for quantitative lipidomic analysis of human samples
Negative control: Not explicitly mentioned

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