AML cell lines and HEK293T cells were maintained under standard conditions with up to 0.01% DMSO. Cell line identity was verified using the Multiplex Cell Authentication Test (Multiplexion, Friedrichshafen, Germany) or the Human Cell Line Authentication Service (Eurofins Genomics GmbH, Ebertsberg, Germany). All cell lines were routinely tested for mycoplasma contamination. AML PDX cells were isolated from the spleen of leukemic mice as described previously56 (link)
and cultured in StemPro34 SFM (Thermo Fisher Scientific, Waltham, Massachusetts, USA) supplemented with provided nutrient supplement, 2% FBS, 1% penicillin/streptomycin and human FLT3 ligand, human thrombopoietin, human interleukin 3, and human stem cell factor (PeproTech, Princeton, New Jersey, USA; 10 ng/mL each). Murine cell lines were a gift from Stephen M. Sykes and were generated and cultured as described previously57 (link)
(liquid culture) or in methylcellulose medium (MethoCult GF M3434, Stem Cell Technologies, Vancouver, Canada). Palbociclib (PD-0332991) and LIMKi3 (CAS number 1338247-35-0) were obtained from Selleck (Munich, Germany) or Merck Millipore (Burlington, Massachusetts, USA), respectively.
and cultured in StemPro34 SFM (Thermo Fisher Scientific, Waltham, Massachusetts, USA) supplemented with provided nutrient supplement, 2% FBS, 1% penicillin/streptomycin and human FLT3 ligand, human thrombopoietin, human interleukin 3, and human stem cell factor (PeproTech, Princeton, New Jersey, USA; 10 ng/mL each). Murine cell lines were a gift from Stephen M. Sykes and were generated and cultured as described previously57 (link)
(liquid culture) or in methylcellulose medium (MethoCult GF M3434, Stem Cell Technologies, Vancouver, Canada). Palbociclib (PD-0332991) and LIMKi3 (CAS number 1338247-35-0) were obtained from Selleck (Munich, Germany) or Merck Millipore (Burlington, Massachusetts, USA), respectively.
