Murine Model Characteristics for Immunology

Six-week-old female C57BL/6 mice were purchased from NCI or Charles River Laboratories. B6.129S2-CD8a^tm1Mak/J(CD8-deficient), B6.129S7-Ifng^tm1Ts/J (IFN-γ-deficient) and C57BL/6-Pfp^tm1Sdz (perforin-deficient) mice were purchased from Jackson Laboratories. B6.129P2-H2-Kb^tm1H2-Db^tm1N12 (MHCI-deficient) mice were purchased from Taconic. gBT-I TCR transgenic mice specific for the glycoprotein B epitope gB(498–505)45 (link) were provided by F.R. Carbone and W.R. Heath and bred in our facility to C57BL/6-Ly5.2Cr mice (CD45.1+) (NCI). Mgl2+/DTR-GFP (Mgl2DTR/GFP) animals have been previously described13 (link). All procedures used in this study complied with federal and institutional policies of the Yale Animal Care and Use Committee.

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Shin H., Kumamoto Y., Gopinath S, & Iwasaki A. (2016). CD301b+ dendritic cells stimulate tissue-resident memory CD8+ T cells to protect against genital HSV-2. Nature Communications, 7, 13346.

Publication 2016

B6.129S2-CD8atm1Mak/J B6.129S7-Ifngtm1Ts/J

Animal C57bl mice Epitope Female Glycoprotein Ifn γ Mice Perforin River Transgenic mice

Corresponding Organization : Howard Hughes Medical Institute

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Variable analysis

independent variables

Mouse genotypes: C57BL/6, B6.129S2-CD8atm1Mak/J (CD8-deficient), B6.129S7-Ifngtm1Ts/J (IFN-γ-deficient), C57BL/6-Pfptm1Sdz (perforin-deficient), and B6.129P2-H2-Kbtm1H2-Dbtm1N12 (MHCI-deficient)
GBT-I TCR transgenic mice specific for the glycoprotein B epitope gB(498–505)

dependent variables

Unspecified

control variables

Sex: Female
Age: Six-week-old
Mouse source: NCI, Charles River Laboratories, and Jackson Laboratories

positive controls

GBT-I TCR transgenic mice

negative controls

C57BL/6 mice

Annotations

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