Atherosclerosis Development in Ldlr-/- Mice

Mice were housed under standard conditions and given free access to food and water. Female Ldlr^−/− mice were lethally irradiated and transplanted with Wt, Cd36^−/− and Msr^−/− bone marrow as previously described [4] (link). After a recovery period of 9 weeks, the mice were given a HFC diet for 3 months (n = 9 Wt-tp mice, n = 8 for both Cd36^−/−-tp and Msr1^−/−-tp mice), containing 21% milk butter, 0.2% cholesterol, 46% carbohydrates and 17% casein. Collection of blood and specimens, biochemical determination of lipids in plasma and liver, liver histology, RNA isolation, cDNA synthesis and qPCR, aminotransferases, oxysterols and auto-antibody titers against IgG and IgM antibodies to CuOx-LDL and MDA-LDL, as well as T15id+ IgM were determined as previously described [4] (link).

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Bieghs V., Verheyen F., van Gorp P.J., Hendrikx T., Wouters K., Lütjohann D., Gijbels M.J., Febbraio M., Binder C.J., Hofker M.H, & Shiri-Sverdlov R. (2012). Internalization of Modified Lipids by CD36 and SR-A Leads to Hepatic Inflammation and Lysosomal Cholesterol Storage in Kupffer Cells. PLoS ONE, 7(3), e34378.

Publication 2012

Aminotransferases Antibodies igm Blood Bone marrow Butter Carbohydrates Casein Cdna Cholesterol Diet Female Food Igg antibody Isolation Ldlr Lipids Liver Mice Milk Oxysterols Plasma Synthesis

Corresponding Organization :

Other organizations : Maastricht University, Institut Pasteur de Lille, Inserm, University of Bonn, Cleveland Clinic Lerner College of Medicine, Austrian Academy of Sciences, Medical University of Vienna, University Medical Center Groningen, University of Groningen

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Variable analysis

independent variables

Bone marrow transplant type (Wt, Cd36-/-, Msr-/-)

dependent variables

Plasma lipid levels
Liver lipid levels
Liver histology
Gene expression (RNA/qPCR)
Aminotransferases
Oxysterols
Auto-antibody titers against IgG and IgM antibodies to CuOx-LDL and MDA-LDL
T15id+ IgM

control variables

Standard housing conditions
Free access to food and water
Ldlr-/- mice
HFC diet (21% milk butter, 0.2% cholesterol, 46% carbohydrates, 17% casein)
Duration of HFC diet (3 months)

controls

Positive control: Wt bone marrow transplant
Negative controls: Cd36-/- and Msr-/- bone marrow transplants

Annotations

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