Induction of Tumor Cell Invasiveness

C57BL/6 background B16F0 and B16F1 melanoma cells, human hepatocellular carcinoma HepG2 cells, and human breast cancer MCF-7 cells were purchased from China Center for Type Culture Collection (CCTCC, Wuhan, China) and cultured according to their guidelines. The cell lines were authenticated at China Center for Type Culture Collection (Wuhan, China) in June 2014, using short tandem repeat (STR) DNA profiling (ABI 31300xl Genetic Analyzer; Life Technologies). To induce the invasive capacity of non-metastatic tumor cells (B16F0, HepG2, MCF-7), the cells were cultured in presence of T/H/H (TGF-β1, 5 ng/ml, H₂O₂, 100 μM, HOCl, 50 μM) for 10 days [19 (link)]. For the convenience of description, T/H/H-pretreated cells were mentioned as T/H/H-B16F0, T/H/H-HepG2, and T/H/H-MCF-7 cells, respectively.