Measuring Cellular Metabolism Markers

Glucose uptake and lactate production were measured with Glucose Assay Kit II and Lactate Assay Kit II (Biovision) according to manufacturer's instructions. Aliquots of the medium were removed from cell culture and Glucose uptake and lactate production was determined by the concentration difference between samples and blank medium control. Cellular ATP and NADH contents were measured using the ATP Colorimetric Assay Kit and NADH Quantitation Kit (Biovision) respectively as described before [9 (link)].

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Wang P., Song M., Zeng Z.L., Zhu C.F., Lu W.H., Yang J., Ma M.Z., Huang A.M., Hu Y, & Huang P. (2015). Identification of NDUFAF1 in mediating K-Ras induced mitochondrial dysfunction by a proteomic screening approach. Oncotarget, 6(6), 3947-3962.

Publication 2015

Glucose Assay Kit II Lactate Assay Kit II ATP Colorimetric Assay Kit

Assay Cell culture Cellular Colorimetric Glucose Lactate Nadh

Corresponding Organization :

Other organizations : Sun Yat-sen University, Sun Yat-sen University Cancer Center, Sun Yat-sen Memorial Hospital, The University of Texas MD Anderson Cancer Center

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Glucose uptake
Lactate production
Cellular ATP content
Cellular NADH content

control variables

Not explicitly mentioned

controls

Blank medium control used to determine the concentration difference for glucose uptake and lactate production

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