RPMI 1640 complete medium with 10% fetal bovine serum (FBS) (Gemini Bio-Product) was used for cell cultures. Oral squamous carcinoma cells and oral squamous carcinoma stem-like cells (OSCSCs) were isolated from cancer patients with tongue tumors at UCLA (2 (link), 33 (link)–35 (link)). Alpha-MEM (Life Technologies, CA, USA) with 10% FBS was used for OCs and DCs cultures. M-CSF was purchased from Biolegend (CA, USA) and RANKL, GM-CSF, and IL-4 were purchased from PeproTech (NJ, USA) and rh-IL-2 was obtained from NIH-BRB. Human CD3/CD28 T cell activator was purchased from Stem Cell Technologies.
Antibodies for MHC-I, KIR2, KIR3, CD44, CD54, B7H1, CD16, NKG2D, MICA/B, KLGR1, CD45, CD3/16/56, CD8, CD3, CD4, GL3, NKp40, NKp30, NKp44, NKp46, and CD94 were purchased from Biolegend (San Diego, CA, USA). ULBP 1–6 antibodies were purchased from R&D Systems. Propidium iodide (PI) was purchased from Sigma (St. Louis, MO, USA). sAJ2 was prepared as described previously (35 (link)).
Antibodies for MHC-I, KIR2, KIR3, CD44, CD54, B7H1, CD16, NKG2D, MICA/B, KLGR1, CD45, CD3/16/56, CD8, CD3, CD4, GL3, NKp40, NKp30, NKp44, NKp46, and CD94 were purchased from Biolegend (San Diego, CA, USA). ULBP 1–6 antibodies were purchased from R&D Systems. Propidium iodide (PI) was purchased from Sigma (St. Louis, MO, USA). sAJ2 was prepared as described previously (35 (link)).
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