Generation of mAurkc-GFP Fusion Proteins

The generation of mAurkc-Gfp was previously described (Shuda et al., 2009 (link)). The AURKC_v1 cDNA clone was purchased from Genecopeia (#EX-Q0034-M02-B, Rockville, USA). AURKC_v2 was cloned via PCR from AURKC_v1 using a forward primer (5′- GATCGCATGCATGGCTACAG-3′). AURKC_v3 was obtained from Thermo Scientific (#160-002-F-8, Somerset, USA). All cDNA clones were PCR amplified and ligated into pIVT-EGFP (Igarashi et al., 2007 (link)) using SphI and SalI. We performed in vitro transcription with a mMessage mMachine kit (Ambion, #AM1344M) as per the manufacturer's instructions. cRNA was purified using an RNA-Easy purification kit (Qiagen, #74 104, Venlo, Netherlands) and eluted in RNase free H₂O.