Accurately weighed 25mg standard Plumbagin was dissolved in 25 ml of methanol to prepare 1mg/ml solution. Preparation of Test samples: Each 1 gm of sample was taken in conical flask along with 10 ml methanol and kept it overnight. Next day all samples are sonicated and centrifuged. Supernatant liquid is collected and stored in test tube for further use. Application of standard and sample: For spot application, Linomat V sample applicator (CAMAG, 4132 Muttenz, Switzerland) was used and samples solution applied to Silica Gel 60 F254 plate as sharp bands. After that this plate kept aside till the spots get dried. After that the mobile phase, Toluene: Formic acid (9.9:0.1) was poured into Twin trough chamber and chamber covered with lid for 45 minutes to allow saturation. Then lid was removed and plate was inserted into the chamber carefully. Hereafter the plate was allowed to develop until the solvent front had travelled at the distance of 70mm above the base of the plate. After this the plate was taken off from the chamber and dried by using blow dryer and then at 266nm wavelength, detection was done by using CAMAG TLC Scanner III & Win cats 4.02 software. This study was done at CRL (Central Research Laboratory), DMIHER ( Datta Meghe Institute of Higher Education & Research), Sawangi (M), Wardha, Maharashtra.