DNA was extracted from EDTA-anticoagulated blood samples using the MagNA Pure LC system (Roche Diagnostics, Basel, Switzerland) following the manufacturer’s instructions.
Using online search engines such as PubMed, Ensemble, and HuGE navigator, a systematic literature search was conducted to identify SNPs statistically significantly associated with LTPA. The search time frame related to the present study was until 5 August 2019. Keywords and their combinations used in the search: leisure time physical activity, recreational physical activity, genetics, genome-wide association study (GWAS), candidate gene, genotype. In the selection of SNPs, particular attention was focused on the results of the three GWAS [14 (link),15 (link),73 (link)] and a candidate gene study [82 (link)], which were the most relevant in this field.
The literature search identified a total of ten SNPs, and these were genotyped using the MassARRAY platform (Sequenom Inc., San Diego, CA, USA) with iPLEX Gold chemistry in the Mutation Analysis Core Facility (MAF) of the Karolinska University Hospital, Sweden. The MAF conducted validation, concordance analysis, and quality control according to their protocols. The Hardy-Weinberg Equilibrium (HWE) and linkage disequilibrium (LD) structure of the genotyped SNPs were calculated by Haploview software (version 4.2; Broad Institute; Cambridge, MA, USA).
Using online search engines such as PubMed, Ensemble, and HuGE navigator, a systematic literature search was conducted to identify SNPs statistically significantly associated with LTPA. The search time frame related to the present study was until 5 August 2019. Keywords and their combinations used in the search: leisure time physical activity, recreational physical activity, genetics, genome-wide association study (GWAS), candidate gene, genotype. In the selection of SNPs, particular attention was focused on the results of the three GWAS [14 (link),15 (link),73 (link)] and a candidate gene study [82 (link)], which were the most relevant in this field.
The literature search identified a total of ten SNPs, and these were genotyped using the MassARRAY platform (Sequenom Inc., San Diego, CA, USA) with iPLEX Gold chemistry in the Mutation Analysis Core Facility (MAF) of the Karolinska University Hospital, Sweden. The MAF conducted validation, concordance analysis, and quality control according to their protocols. The Hardy-Weinberg Equilibrium (HWE) and linkage disequilibrium (LD) structure of the genotyped SNPs were calculated by Haploview software (version 4.2; Broad Institute; Cambridge, MA, USA).
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