The following primary antibodies were used: Rabbit polyclonal anti-CagA antibody was purchased from Austral Biological (San Ramon, CA, USA). The mouse polyclonal anti-urease antibodies and anti-CagN antibodies were described elsewhere [85] (link), [86] (link). Polyclonal rabbit antibodies recognizing a series of other H. pylori proteins, were raised against peptides corresponding to the following conserved amino acid (aa) residues derived from strain 26695: BabA (aa 126–140: CGGNANGQESTSSTT), SabA (aa 172–186: CAMDQTTYDKMKKLA), OipA (aa 275–282: NYYSDDYGDKLDYK), NapA (aa 105–118: EFKELSNTAEKEGD), Slt (aa 492–505: LRRWLESSKRFKEK), HtrA (aa 90–103:DKIKVTIPGSNKEY), FlaA (aa 93–106: KVKATQAAQDGQTT), GGT (aa 175–188: RQAETLKEARERFL), DupA (aa 551–564: MLNIDSDNQQDNKA), VirB10/CagY (repeat region: VSRARNEKEKKE), Cagδ (aa 32–45: IKATKETKETKKEA), and Rlx2 (aa 131–144: HLVFSIDENSNEKN). Rabbit anti-Rlx1 and anti-CagM antibodies were raised against the entire recombinant Rlx1 or CagM proteins, respectively. All antibodies were affinity-purified and prepared according to standard protocols by Biogenes GmbH (Berlin, Germany). Horseradish peroxidase-conjugated anti-mouse or anti-rabbit polyvalent sheep immunglobulin was used as secondary antibody (DAKO Denmark A/S, DK-2600 Glostrup, Denmark) and blots were developed with ECL Plus Western blot reagents (GE Healthcare, UK limited Amersham Place, UK) [87] (link)–[89] (link).
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