The water vapor transmission test was carried out according to a procedure described previously [34 (link)]. For this purpose, silica gel with indicator (5 g) was put into glass vials (diameter of mouth was 1 cm) and they were subjected to drying (Drying oven SUP-65, Wamed, Warsaw, Poland). Simultaneously, the samples of curdlan-based biomaterials and KALTOSTAT® specimens were immersed in SWF (24 h, room temperature). Then, the vials containing dry silica gel were weighed. The biomaterials were removed from the solution, blotted with tissue paper, and mounted precisely on the mouth of the vials. After 24 h incubation at 37 °C and 95% relative humidity (Heraeus cytoperm 2, Thermo Scientific, Waltham, MA, USA), the biomaterials were removed from the vials and the mass of vials with wet silica gel was measured. The water vapor transmission rate (WVTR) was calculated as follows: WVTR (g/m2/day)=WwWdS
where Ww denotes the weight of the vial with wet gel (g), Wd denotes the weight of the vial with dry gel (g), and S denotes the surface of the vial mouth (m2).
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